Jayeshbhai Chaudhari, Hiep L X Vu Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive sense, single-stranded RNA virus that is known to infect only pigs. The virus emerged in the late 1980s and became endemic in most swine producing countries, causing substantial economic losses to the swine industry. The first reverse genetics system for […]

Hung Q. Luong, Huong T. L. Lai, and Hiep L. X. Vu Luciferase-immunoprecipitation system (LIPS), a liquid phase immunoassay, was used to evaluate antibody responses directed against the structural proteins of PRRSV in pigs that were experimentally infected with virulent PRRSV strains. First, the viral N protein was used as a model antigen to validate the assay. The […]

Jayeshbhai Chaudhari, Chia-Sin Liew, Aspen M Workman, Jean-Jack M Riethoven, David Steffen, Sarah Sillman, Hiep L X Vu Both virulent and live-attenuated porcine reproductive and respiratory syndrome virus (PRRSV) strains can establish persistent infection in lymphoid tissues of pigs. To investigate the mechanisms of PRRSV persistence, we performed a transcriptional analysis of inguinal lymphoid tissue collected from pigs experimentally infected with […]

Haiyan Sun, Aspen Workman, Fernando A Osorio, David Steffen, Hiep L X Vu Modified-live virus (MLV) vaccines are widely used to protect pigs against porcine reproductive and respiratory syndrome virus (PRRSV). However, current MLV vaccines do not confer adequate levels of heterologous protection, presumably due to the substantial genetic diversity of PRRSV isolates circulating in the field. To overcome […]

Kathryn Kimpston-Burkgren, Ignacio Correas, Fernando A Osorio, David Steffen, Asit K Pattnaik, Ying Fang, Hiep L X Vu The minor glycoproteins (GPs) of PRRSV, GP2, GP3, and GP4, form a heterotrimer that is required for viral infectivity, presumably due to its interaction with the key cellular receptor CD163. These 3GPs are encoded by open reading frames (ORFs) 2a, 3 and 4 […]